Peptide Reconstitution Calculator
Tell us what’s in your vial and how much water you added — we’ll show you exactly how many units to draw. Made for beginners.
Peptide Calculator
Three quick steps — no chemistry required
What's in the vial?
How much water did you add?
Your mix is 5000.00 mcg/mL — every 1 unit on the syringe = 50.00 mcg.
What do you want to know?
For 250 mcg per draw
5.0 units
Draw to the 5.0-unit mark (0.050 mL)
U-100 insulin syringe
Pull the plunger to the 5.0-unit mark.
Volume drawn
0.050 mL
Draws per vial
40
Double-check against your research protocol. For laboratory and research use only.
Dilution
Thin an existing solution down to a lower strength
Concentration of the solution you already have
Total mL of solution currently in the vial
The lower concentration you want to dilute down to
C₁V₁ = C₂V₂ — enter the current concentration and volume of your solution, then the target concentration. The calculator will determine how much solvent to add.
Solvent to add
4.000 mL
bacteriostatic water
Final volume
5.000 mL
1 mL + 4.000 mL
Dilution factor
5.0×
1:5.0
Common questions
New to peptides? Start here.
Sterile water with 0.9% benzyl alcohol as a preservative. It inhibits bacterial growth and is the standard solvent for reconstituting lyophilized peptides. Usable for up to 28 days once opened when stored at 2-8°C.
Swab the vial stopper with alcohol. Draw your desired amount of BAC water into a syringe. Dispense it slowly against the inside wall of the vial—not directly onto the powder. Gently swirl until fully dissolved. The solution should be clear and colorless.
A standard U-100 insulin syringe reads 100 units per 1 mL. 10 units = 0.1 mL, 50 units = 0.5 mL. The calculator above converts your amount to syringe units automatically.
It depends on your target concentration. More water = more dilute (easier to measure small amounts). Less water = more concentrated (fewer draws to use the vial). Common amounts are 1–3 mL. Try a couple of values in the calculator to compare.
Refrigerate at 2-8°C. Do not freeze reconstituted solutions. Protect from light. Most are stable 2–4 weeks when stored properly. Unreconstituted peptides can be stored frozen for longer-term storage.
1 mg = 1,000 mcg. Vial contents are typically labeled in mg. Smaller measurements are often expressed in mcg for precision. The calculator handles both and converts between them.