Research Use Only
Peptide Reconstitution Calculator
Calculate reconstitution volumes, solution concentrations, and draw amounts for lyophilized peptide research compounds.
Reconstitution
Total mg in the entire vial, as printed on the label
Volume of bacteriostatic water you will add to the vial
How much peptide you want in a single draw
Concentration
2500.00 mcg/mL
2.50 mg/mL
Volume per draw
0.100 mL
10.0 units on U-100 syringe
Draws per vial
20
at 250 mcg each
U-100 Syringe
Draw to the 10.0-unit mark (0.10 mL) for 250 mcg per draw.
Volume & Amount
The amount of peptide per mL after reconstitution — use the result from above or check your vial label
The amount of peptide you want in a single draw
How many times per day a draw is taken from the vial
Results
Draw volume
0.100 mL
10.0 units
Per draw
250.00 mcg
0.250 mg
Daily total
250.00 mcg
1× per day
Weekly total
1750.00 mcg
1.75 mg
Verify calculations against your research protocol. For reference only.
Dilution
Concentration of the solution you already have
Total mL of solution currently in the vial
The lower concentration you want to dilute down to
C₁V₁ = C₂V₂ — enter the current concentration and volume of your solution, then the target concentration. The calculator will determine how much solvent to add.
Solvent to add
4.000 mL
bacteriostatic water
Final volume
5.000 mL
1 mL + 4.000 mL
Dilution factor
5.0×
1:5.0
Reference
Sterile water with 0.9% benzyl alcohol as a preservative. It inhibits bacterial growth and is the standard solvent for reconstituting lyophilized peptides. Usable for up to 28 days once opened when stored at 2-8°C.
Swab the vial stopper with alcohol. Draw your desired amount of BAC water into a syringe. Dispense it slowly against the inside wall of the vial—not directly onto the powder. Gently swirl until fully dissolved. The solution should be clear and colorless.
A standard U-100 insulin syringe reads 100 units per 1 mL. 10 units = 0.1 mL, 50 units = 0.5 mL. The calculators above convert mL to syringe units automatically.
It depends on your target concentration. More water = more dilute (easier to measure small amounts). Less water = more concentrated (fewer draws to use the vial). Common amounts are 1–3 mL. Use the reconstitution calculator above to compare.
Refrigerate at 2-8°C. Do not freeze reconstituted solutions. Protect from light. Most are stable 2–4 weeks when stored properly. Unreconstituted peptides can be stored frozen for longer-term storage.
1 mg = 1,000 mcg. Vial contents are typically labeled in mg. Smaller measurements are often expressed in mcg for precision. All calculators on this page handle both and convert between them.